首页>
外文期刊>cell and tissue research
>Immuno-localization of ferritin polypeptides in oocytes and somatic tissue of the freshwater snailsLymnaea stagnalisL. andPlanorbarius corneusL.
【24h】
Immuno-localization of ferritin polypeptides in oocytes and somatic tissue of the freshwater snailsLymnaea stagnalisL. andPlanorbarius corneusL.
展开▼
机译：Immuno-localization of ferritin polypeptides in oocytes and somatic tissue of the freshwater snailsLymnaea stagnalisL. andPlanorbarius corneusL.
Antibodies were raised in rabbits against the 19000 Mr and 24000 Mr polypeptides of snail ferritin fromLymnaea stagnalisL. Anti-24000 Mr polypeptide antibodies were purified by an affinity-purification step and were made monospecific for their antigen by preabsorption with the 19000 Mr antigen. These purified antibodies were then used for in situ detection of their respective antigens by the indirect immunofluorescence method. The 19000 Mr polypeptide was found widely distributed in tissues of both pulmonate snails investigated (Lymnaea stagnalisL. andPlanorbarius corneusL.) with the most intense antigen-directed fluorescence in certain connective tissue cells, secretory cells of the midgut gland and Sertoli cells and epithelia of the gonadal acini. In contrast, the 24000 Mr polypeptide could be detected only in yolk platelets of vitellogenic oocytes. The results indicate that yolk and somatic cell ferritins differ in immunoreactivity and structure and, accordingly may differ in function.
展开▼
