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>Human hypertensive placenta contains an increased amount of Na, K‐ATPase with higher affinity for cardiac glycosides
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Human hypertensive placenta contains an increased amount of Na, K‐ATPase with higher affinity for cardiac glycosides
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机译：Human hypertensive placenta contains an increased amount of Na, K‐ATPase with higher affinity for cardiac glycosides
ABSTRACTPlacentas of women suffering from pregnancy‐induced hypertension (PIH) were found to contain a greater amount of Na,K‐ATPase molecules, estimated from anthroyl ouabain binding, than normotensive individuals. Both the microsomal fraction of placental cells and purified Na,K‐ATPase showed an increased affinity for the specific inhibitor ouabain which, in the case of the microsomes, bound with a dissociation constant of 0.9 nM as compared with 3.4 nM in the controls. Likewise, the dissociation constant of the ouabain complex with purified Na,K‐ATPase was about 3.5 times lower in the hypertensive patients. The differences are apparently caused by a different microenvironment of the ouabain‐binding site, as reflected in the quantum yield of bound anthroyl ouabain. If an endogenous digitalis‐like factor is present in the body fluids to regulate Na,K‐ATPase activity, the present results render its role qu
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