The effect of different temperatures on bud formation in excised leaf fragments and in stem segments ofHeloniopsis orientalis, a monocotyledonous plant, was investigated in light and in darkness. The optimal temperature for bud formation was 21°C to 25°C. 16°C pretreatment for 7 to 21 days promoted bud formation in leaf segments. 30°C pretreatment for 7 days or more reduced the number of buds in both young etiolated and mature green leaf segments but not in young green leaf segments. In young green leaf segments grown in darkness, however, 30°C pretreatment reduced the number of buds. Inhibition of bud formation due to high temperature could not be reversed b
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