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>Comparison of skin‐prick test assay and reverse enzyme immunoassay competition (REINA‐C) for biological activity of allergens
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Comparison of skin‐prick test assay and reverse enzyme immunoassay competition (REINA‐C) for biological activity of allergens
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机译:Comparison of skin‐prick test assay and reverse enzyme immunoassay competition (REINA‐C) for biological activity of allergens
SummaryAnin vitrotechnique for measuring the relative potency of allergenic extracts has been developed and compared with the dose‐response skin‐prick test, Thein vitrotechnique is a modification of an EL1SA based on the method of antigen capture using polystyrene microtitre wells as solid phase (REINA‐compelition) and is also based on a doseresponse. Both methods have been applied to allergen extracts ofDermatophagoides pteronyssinus. Phleum pratense, Secale cereale, Lolium perenne, Plantigo lanceolata, Artemisia vulgaris, Salsola kali and Parietaria judaica.For each allergen, the slope of the regression log‐log dose response lines displayed by skin‐prick test and REINA‐C does not show a statistically significant difference, being parallel (P<0.001), and indicating that the allergen ligand binding kinetics are identical in b
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