AbstractMAP1a is a microtubule‐associated protein with an apparent molecular weight of 360 kDa that is found in the axonal and dendritic processes of neurons. Two monoclonal anti‐MAP1a antibodies, anti‐A and anti‐BW6, revealed different epitope distributions in the adult mouse cerebellum. Anti‐A stained Purkinje and granule cells uniformly throughout the cerebellum. In contrast, anti‐BW6 selectively stained the dendrites of a subset of Purkinje cells, revealing parasagittal bands of immunoreactivity in the molecular layer. The compartmentation of the BW6 epitope was compared to the Purkinje cells as revealed by immunostaining with anti‐zebrin II, a well known antigen expressed selectively by bands of Purkinje cells. The anti‐BW6 staining pattern was complementary to the zebrin II bands, the zebrin II‐Purkinje cells having BW6+dendrites. These results demonstrate that MAP1a is present in two forms in the mouse cerebellum, one of which is segregated into parasagittal bands. This may indicate a unique MAP1a isoform or may reflect differences in the metabolic states of Purkinje cell classes, and regional differences i
展开▼
