AbstractDimethylsulfoxide (DMSO) converts almost all of the undifferentiated murine erythroleukemia cells (MEL or Friend cells, clone 745A) in a culture to differentiated cells that contain high levels of hemoglobin and that stop growing after a limited number of cell divisions. Contrary to other reports—that amiloride strongly inhibits DMSO‐induced differentiation in MEL cells—in this laboratory, inhibition by amiloride, tested with DMSO over a range of concentrations in two kinds of media and at various cell densities, was found to be only weak or absent. Similarly, amiloride did not inhibit induction by N, N′‐hexamethylene bis‐acetamide (HMBA). As expected from previous findings with other cell systems, amiloride inhibited protein synthesis and cell mul
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