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>Isolation and characterization of alkaline phosphatase‐constitutive variants from chinese hamster ovary cells (CHO‐K1)
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Isolation and characterization of alkaline phosphatase‐constitutive variants from chinese hamster ovary cells (CHO‐K1)
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机译:Isolation and characterization of alkaline phosphatase‐constitutive variants from chinese hamster ovary cells (CHO‐K1)
AbstractPure clones with high alkaline phosphatase (ALP) activity were isolated from Chinese hamster ovary (CHO‐K1) cells which showed no detectable ALP activity. ALP‐positive cells appeared at the frequency of 10−4in the N‐methyl‐N'‐nitro‐N‐nitrosoguanidine‐treated cell population.With respect for cellular morphology, plating efficiency and several other enzymatic activities, no distinct difference was found between the original CHO‐K1 cells and its ALP‐positive variants, although some alterations in karyotype were found.Levels of ALP activity in these clones was stably maintained during serial cultivation. Some of the enzymological properties of ALP in the isolated clones were similar to th
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