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An electroanalytical method for estimation of fish freshness using a flow‐injection system with some immobilized enzyme reactors
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机译：An electroanalytical method for estimation of fish freshness using a flow‐injection system with some immobilized enzyme reactors
AbstractA flow‐injection system is proposed for the estimation offish freshness. The 5′‐adenylic acid deaminase reactor, alkaline phosphatase reactor, and nucleoside phosphorylase‐xanthine oxidase coimmobilized reactor were incorporated at fixed positions in a flow system, which was based on the splitting of the flow after sample injection and subsequent confluence before reaching the peroxidase electrode. Because each channel has a different residence time, two peaks were obtained. The first peak corresponded to the total of hypoxanthine and inosine, and the second peak to the total of hypoxanthine, inosine, inosine‐5′‐monophosphate, and adenosine‐5′‐monophosphate. The index of fish freshness,K, is estimated bydocumentclass{article}pagestyle{empty}begin{document}$$ K = (S_2 /S_1)(i_1 /i_2),{rm x},{rm 100} $$end{document}where s1and s2represent the sensitivity (nA mM−1) andi1andi2represent the peak current (nA) of the first and second peaks, respectively. The measurements could be performed at a rate of 15 samples per hour with s
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