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Regeneration andAgrobacterium-mediated transformation ofForsythiaxintermedia“Spring Glory”

机译:Regeneration andAgrobacterium-mediated transformation ofForsythiaxintermedia“Spring Glory”

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摘要

Internode explants ofin vitroplants ofForsythia x intermedia“Spring Glory” were transformed with thegusandnpt IIgenes after inoculation with theA. tumefaciensstrain EHA 101 harbouring the plasmid pFAJ3000. Shoot organogenesis took place from callused edges of explants. The first transformed buds were detected 4 to 6 weeks after transfer on regeneration medium, containing 25 mg/l kanamycin as selective agent. An average of 1 of explants regenerated transgenic sho

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