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首页> 外文期刊>Experimental Animals >Lumbar Intervertebral Disc Puncture under C-arm Fluoroscopy: a New Rat Model of Lumbar Intervertebral Disc Degeneration
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Lumbar Intervertebral Disc Puncture under C-arm Fluoroscopy: a New Rat Model of Lumbar Intervertebral Disc Degeneration

机译：C臂透视下腰椎间盘穿刺：腰椎间盘退变的新大鼠模型

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摘要

To establish a minimally invasive rat model of lumbar intervertebral disc degeneration (IDD) to better understand the pathophysiology of the human condition. The annulus fibrosus of lumbar level 4-5 (L4-5) and L5-6 discs were punctured by 27-gauge needles using the posterior approach under C-arm fluoroscopic guidance. Magnetic resonance imaging (MRI), histological examination by hematoxylin and eosin (HE) staining, and reverse transcription polymerase chain reaction (RT-PCR) were performed at baseline and 2, 4, and 8 weeks after disc puncture surgery to determine the degree of degeneration. All sixty discs (thirty rats) were punctured successfully. Only two of thirty rats subjected to the procedure exhibited immediate neurological symptoms. The MRI results indicated a gradual increase in Pfirrmann grade from 4 to 8 weeks post-surgery (P<0.05), and HE staining demonstrated a parallel increase in histological grade (P<0.05). Expression levels of aggrecan, type II collagen (Col2), and Sox9 mRNAs, which encode disc components, decreased gradually post-surgery. In contrast, mRNA expression of type I collagen (Col1), an indicator of fibrosis, increased (P<0.05). The procedure of annular puncture using a 27-gauge needle under C-arm fluoroscopic guidance had a high success rate. Histological, MRI, and RT-PCR results revealed that the rat model of disc degeneration is a progressive pathological process that is similar to human IDD.
机译：建立腰椎间盘退行性变（IDD）的微创大鼠模型，以更好地了解人类病理生理学。腰椎 4-5 水平 （L4-5） 和 L5-6 椎间盘的纤维环在 C 臂透视引导下使用后路入路用 27 号针刺穿。在基线和椎间盘穿刺手术后 2、4 和 8 周进行磁共振成像 （MRI）、苏木精和伊红 （H&E） 染色组织学检查和逆转录聚合酶链反应 （RT-PCR） 以确定变性程度。所有60个圆盘（30只大鼠）均成功刺破。在接受手术的30只大鼠中，只有两只表现出即时的神经系统症状。MRI结果显示，术后4周至8周Pfirrmann分级逐渐升高（P<0.05），H&E染色显示组织学分级平行升高（P<0.05）。编码椎间盘成分的聚集聚糖、II 型胶原 （Col2） 和 Sox9 mRNA 的表达水平在术后逐渐下降。相比之下，纤维化指标I型胶原（Col1）的mRNA表达增加（P<0.05）。在C臂透视引导下使用27号针头进行环形穿刺的手术成功率很高。组织学、MRI和RT-PCR结果显示，大鼠椎间盘退行性变模型是一个进行性病理过程，与人类IDD相似。

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