SummaryUp to 70 of mouse spleen cells expressing H‐2Kkalloantigens reacted with the monoclonal anti‐H‐2Kkantibody 11‐4.1 in an indirect rosetting assay. When the cells that rosetted with the monoclonal antibody 11‐4.1 were removed by density centrifugation, the residual unreactive cells reacted with the polyclonal anti‐H‐2Kkalloantiserum D23 but not with the monoclonal antibody 11‐4.1 indicating the existence of two cell populations. In sequential immunoprecipitation experimens, the glycoprotein fraction of NP40 extracts from H‐2Kkcells, after removal of antigens reacting with the monoclonal antibody 11‐4.1 still contained structures reactive with the alloantiserum. Therefore, there are at least two antigenically distinct H‐2Kkmolecules which are differentially expressed on two subpopula
展开▼
