AbstractThe presence of aluminum in a minimal mineral medium, with citrate as the sole source of carbon, elicited the production of an exocellular lipid‐rich residue inPseudomonas fluorescens. Although aluminum affected the growth rate, no apparent diminution in cellular yield was observed in medium supplemented with up to 3 mM of the trivalent metal. While a 10 reduction in cellular yield was recorded in medium with 7.5 mM aluminum, a decrease of 69 was reported in medium supplemented with 50 mM aluminum at stationary phase of growth. The wet weight of the pellet ranged from 5.9 to 70.3 mg mL−1of culture in medium supplemented with 1–30 mM aluminum. The trivalent metal was immobilized in this precipitate and associated with phosphate moieties. Both neutral lipids and phospholipids constituted the pellet. More than 90 of the weight of the wet pellet was lost following lyophilization. The percentage of aluminum immobilized increased with the concentration of trivalent metal in the medium. Preconditioned cells showed an improved growth rate, and the cellular yield was slightly higher than for unadapted
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