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外文期刊>european journal of neuroscience
>In vitroandIn vivoAnalysis of a Rat Bipotential O – 2A Progenitor Cell Line Containing the Temperature‐sensitive Mutant Gene of the SV40 Large T Antigen
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In vitroandIn vivoAnalysis of a Rat Bipotential O – 2A Progenitor Cell Line Containing the Temperature‐sensitive Mutant Gene of the SV40 Large T Antigen
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机译：In vitroandIn vivoAnalysis of a Rat Bipotential O – 2A Progenitor Cell Line Containing the Temperature‐sensitive Mutant Gene of the SV40 Large T Antigen
AbstractPrimary cultures from neonatal rat optic nerve contain pluripotential O – 2A progenitor cells that are capable of differentiating into oligodendrocytes, type‐2 astrocytes or adult O – 2A progenitors (O – 2Aadult). Since primary optic nerve cultures contain a mixture of glial cell types of which only a small number are O – 2A progenitors, experiments on cell lineage and differentiation carried out using these cultures are both intrinsically limited and difficult to interpret. Ideally, cells from a clonal cell population would provide the optimal starting material for biological studies. In this paper we describe the creation of an O – 2A progenitor cell line using a retrovirus carrying a temperature‐sensitive mutant SV40 large T antigen gene. This cell line has provided sufficient numbers of cells to allow analysis of theirin vitroproperties and their behaviour following transplantation into anin vivoenvironment. At the non‐permissive temperature (39°C), these cells differentiate into oligodendrocytes and type‐2 astrocytes in a similar fashion to O – 2A progenitor cells from primary cultures (O – 2Aprim). When grown in media containing platelet‐derived growth factor and basic fibroblast growth factor, the cell numbers can be expanded in culture without differentiating, consistent with the behaviour of O – 2Aprimprogenitor cells. By exploiting this property, it has been possible to culture large numbers of O – 2A progenitors forin vivoanalysis. In this study we have shown that transplantation of this O – 2A cell line into glia‐free areas in adult rat spinal cord results in differentiation of a proportion of cells into oligodendrocytes which are capable of myelinating axons. Furthermore, differentiation of O – 2A cells into astrocytes was also observed, indicating that the bipotentiality of these cellsin vi
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