AbstractA variety of affinity cross‐flow filtration (ACFF) experiments were conducted to evaluate the technique as a means of effectively separating biomolecules. Agarose particles, which contained a specific affinity ligand for the targeted protein, were used as “affinity escorts” in the ACFF process. Both conventional agarose particles (40–150; μm) and small agarose particles (Superose, 11–15 μm.) were used as the basis for affinity escorts. Batch ACFF washing experiments were conducted in both constant‐volume and volume‐reduction modes and compared with model predictions. The different affinity ligand‐adsorbate systems studied include Cibacron Blue and human serum albumin, Cibacron Blue and lysozyme, protein A and immunoglobulin G, and concanavalin A and horse
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