GC-MS volatolomic approach to study the antimicrobial activity of the antarctic bacterium Pseudoalteromonas sp. TB41

RomoliR.; PapaleoM.C.; DePascaleD.TutinoM.L.MichaudL.LoGiudiceA.FaniR.BartolucciG.

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GC-MS volatolomic approach to study the antimicrobial activity of the antarctic bacterium Pseudoalteromonas sp. TB41

机译：GC-MS volatolomic approach to study the antimicrobial activity of the antarctic bacterium Pseudoalteromonas sp. TB41

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Many bacteria produce a wide range of volatile info-chemicals compounds (mVOCs) that constitute an important regulatory factor in the interrelationships among different organisms in microbial ecosystems. It has been shown that Antarctic bacteria isolated from three different sponge species, by producing mVOCs, are able to inhibit specifically the growth of Burkholderia cepacia complex (Bcc) strains (i.e. opportunistic pathogens of cystic fibrosis patients) as demonstrated by cross-streaking inhibition assays. This study reports a metabolomics approach to investigate the volatile profile of both the Antarctic sponge-associated Pseudoalteromonas sp. TB41 (P-sp-TB41) and Burkholderia cenocepacia strain LMG16654 (Bc-LMG16654) under aerobic conditions. Solid phase micro extraction (SPME) in head space of biological samples allowed an in vivo sampling of mVOCs with minimal specimen disturbance. The SPME fiber was termically desorbed in the injection port of gas chromatography-mass spectrometer (GC-MS) system setted in EI scan mode. The raw data were processed using both an automated mass spectra deconvolution and identification system and a metabolomic approach, which allowed a selection of 30 compounds presumably responsible for the inhibition of Bc-LMG16654 growth. The results obtained from samples prepared under cross-streaking conditions also suggest that the presence of Bc-LMG16654 cells neither interferes with the production of mVOCs nor induces the synthesis of different mVOCs. The employing of mass spectrometry played a key role in tuning the experimental system and in the evaluation of results. The use of this approach to study the interaction, in aerobic condition, among other Antarctic bacteria and Bcc and the possibility to extend this approach to other pathogen-antagonist relationship, is currently in progress.

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《Metabolomics: Official journal of the Metabolomic Society》 |2014年第1期|42-51|共10页
作者
RomoliR.; PapaleoM.C.; DePascaleD.TutinoM.L.MichaudL.LoGiudiceA.FaniR.BartolucciG.;
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作者单位

Mass Spectrometry Center (CISM), University of Florence, Viale G. Pieraccini 6, 50139 Florence;

Laboratory of Microbial and Molecular Evolution, Department of Evolutionary Biology, University of;

Institute of Protein Biochemistry, National Research Council, Naples, ItalyDepartment of Chemical Sciences, University of Naples Federico II, Naples, ItalyDepartment of Biological and Environmental Science, University of Messina, Messina, ItalyDepartment of Neurosciences, Psychology, Drug Research and Child Health, Section of Pharmaceutical;

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正文语种英语
中图分类生理学;
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Chemical communication; Cystic fibrosis; Genetic disease; Microbiological techniques; Sample preparation; Statistical analysis;

GC-MS volatolomic approach to study the antimicrobial activity of the antarctic bacterium Pseudoalteromonas sp. TB41

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