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首页> 外文期刊>Biochemistry >A pH Switch Regulates the Inverse Relationship between Membranolytic and Chaperone-like Activities of HSP-1/2, a Major Protein of Horse Seminal Plasma
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A pH Switch Regulates the Inverse Relationship between Membranolytic and Chaperone-like Activities of HSP-1/2, a Major Protein of Horse Seminal Plasma

机译：pH 开关调节马精浆的主要蛋白质 HSP-1/2 的膜溶解和伴侣样活性之间的反比关系

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HSP-1/2, a major protein of horse seminal plasma binds to choline phospholipids present on the sperm plasma membrane and perturbs its structure by intercalating into the hydrophobic core, which results in an efflux of choline phospholipids and cholesterol, an important event in sperm capacitation. HSP-1/2 also exhibits chaperone-like activity (CLA) in vitro and protects target proteins V) against various kinds of stress. In the present study we show that HSP-1/2 exhibits destabilizing activity toward model supported and cell membranes. The membranolytic activity of HSP-1/2 is found to be pH dependent, with lytic activity being high at mildly acidic pH (6.0-6.5) and low at mildly basic pH (8.0-04 8.5). Interestingly, the CLA is also found to be pH dependent, with high activity at mildly basic pH and low activity at mildly acidic pH. Taken together the present studies demonstrate that the membranolytic and chaperone-like activities of HSP-1/2 have an inverse relationship and are regulated via a pH switch, which is reversible. The higher CIA observed at mildly basic pH could be correlated to an increase in surface hydrophobicity of the protein. To the best of our knowledge, this is the first study reporting regulation of two different activities of a chaperone protein by a pH switch.
机译：HSP-1/2 是马精浆的主要蛋白质，与精子质膜上存在的胆碱磷脂结合，并通过嵌入疏水核心来扰乱其结构，从而导致胆碱磷脂和胆固醇的外流，这是精子获能的重要事件。HSP-1/2 在体外还表现出伴侣样活性 （CLA），并保护靶蛋白 V） 免受各种压力。在本研究中，我们发现 HSP-1/2 对模型支持膜和细胞膜表现出不稳定活性。发现 HSP-1/2 的膜溶解活性是 pH 依赖性的，在弱酸性 pH （6.0-6.5） 下裂解活性高，在弱碱性 pH （8.0-04 8.5） 下低。有趣的是，共轭亚油酸也被发现具有pH依赖性，在温和碱性pH值下活性高，在弱酸性pH值下活性低。综上所述，本研究表明，HSP-1/2的膜溶解和伴侣样活性呈反比关系，并通过可逆的pH开关进行调节。在温和碱性pH值下观察到的较高CIA可能与蛋白质表面疏水性的增加有关。据我们所知，这是第一项报告通过pH开关调节伴侣蛋白的两种不同活性的研究。

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