AbstractSubmicrogram concentrations (0.04‐‐0.29 μM) of the microfilament disrupting agents cytochalasins D, E, and B (CD, CE, CB) were shown to inhibit secretagogue‐stimulated14C‐aminopyrine accumulation (AP) in isolated rat gastric mucosal parietal cells. The microtubule disrupting agent colchicine had little influence on AP accumulation. Histamine‐ and dibutyryl cyclic AMP (DbcAMP)‐stimulated AP accumulation was inhibited with an order of potency CD>CE ≈︁ CB. CB inhibition of these secretagogue actions was, however only approximately 65‐‐70 of the maximal stimulated response, whereas CD and CE caused 100 inhibition. On the other hand, carbamylcholine‐stimulated AP accumulation was inhibited 100 by all cytochalasins tested with an order of potency CD ≈︁ CE>CB. These data are discussed in relation to acid secretagogue‐induced morphological changes involving actin filament or
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