Interaction Between Antibodies and Hapten-Protein Conjugates of Different Composition: Theoretical Predictions and Experimental Data

AnatoliyV. Zherdev; OlgaG. Romanenko; BorisB. Dzantiev

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Interaction Between Antibodies and Hapten-Protein Conjugates of Different Composition: Theoretical Predictions and Experimental Data

机译：Interaction Between Antibodies and Hapten-Protein Conjugates of Different Composition: Theoretical Predictions and Experimental Data

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摘要

Mathematical models of competitive ELISAs with labelled antibody and with labelled antigen taking into account bivalent interactions between antibodies and hapten-protein conjugates were developed and analyzed. It was shown that in the kinetic model of the immunochemical reaction the conjugate composition influenced the amplitude of detected signal but not ELISA sensitivity. In the equilibrium model decreased sensitivity correlated with bivalent complexes formation.

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《journal of immunoassay》 |1997年第1期|67-95|共页
作者
AnatoliyV. Zherdev; OlgaG. Romanenko; BorisB. Dzantiev;
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正文语种英语
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immune complexes; affinity of antibodies; hapten-protein conjugates; ELISA; testosterone; 2,4-dichlorophenoxyacetic acid;

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Interaction Between Antibodies and Hapten-Protein Conjugates of Different Composition: Theoretical Predictions and Experimental Data

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