AbstractAdipocytes were isolated from the Wistar and Sprague‐Dawley strains of rats of different ages. An impermeable reagent, mercury‐3H dextran, was used to quantitate the sulfhydryl groups on the surface of these cells. The application of this reagent after the pre‐reduction of cells with 2‐mercaptoethanol yielded a measure of the total sulfhydryl and disulfide groups located there. Aging was found to significantly decrease the ratio of the number of exofacial sulfhydryl groups to the total number of exofacial sulfhydryl and disulfide
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