Stability of A+U-rich element binding factor 1 (AUF1)-binding messenger ribonucleic acid correlates with the subcellular relocalization of AUF1 in the rat uterus upon estrogen treatment

Arao  Y; Kikuchi  A; Kishida  MYonekura  MInoue  AYasuda  SWada  SIkeda  KKayama  F

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Stability of A+U-rich element binding factor 1 (AUF1)-binding messenger ribonucleic acid correlates with the subcellular relocalization of AUF1 in the rat uterus upon estrogen treatment

机译：Stability of A+U-rich element binding factor 1 (AUF1)-binding messenger ribonucleic acid correlates with the subcellular relocalization of AUF1 in the rat uterus upon estrogen treatment

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The nucleocytoplasmic shuttling protein, A + U-rich element binding factor 1 (AUF1), is one of the RNA-binding proteins that specifically bind adenylateuridylate rich elements (AREs) in mRNA 3'-untranslated regions ( UTRs), and acts as a regulator of ARE-mediated mRNA degradation in the cytoplasm. We previously reported that in the female rat uterus, the levels of specific AUF1 isoform mRNAs (p40/p45) were increased by 17beta-estradiol (E2) treatment. Therefore, we examined the role of AUF1 in the regulation of E2-mediated mRNA turnover in the rat uterus. We identified ABIN2 and Ier2/pip92 mRNAs as candidate targets of AUF1 in the rat uterus. We found that AUF1-binding elements were present in the 3'-UTR of both mRNAs and that the 3'-UTRs functioned as mRNA turnover regulatory elements. In the ovariectomized rat uterus, the nucleocytoplasmic localization of AUF1p40/p37 isoform proteins was regulated by E2. We also found that cytoplasmic AUF1-bound mRNA levels changed coincidentally with the cytoplasmic levels of AUF1p40/p37. Finally, we confirmed that the subcellular localization of AUF1p40 controlled the stability of target mRNAs in vitro, such that cytoplasmically localized AUF1p40 led to marked mRNA stabilization, whereas nuclear-localized AUF1p40 stabilized target mRNA only slightly. These results suggested that E2-inducible ARE-containing gene transcripts are regulated, at least in part, via mRNA stabilization through the nucleocytoplasmic relocalization of AUF1.

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《Molecular Endocrinology》 |2004年第9期|2255-2267|共13页
作者
Arao Y; Kikuchi A; Kishida MYonekura MInoue AYasuda SWada SIkeda KKayama F;
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Jichi Med Sch, Ctr Community Med, Dept Environm Med, Minami Kawachi, Tochigi 3290498, Japan;

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正文语种英语
中图分类内分泌腺疾病及代谢病;
关键词
CYTOPLASMIC SHUTTLING PROTEIN; NUCLEAR RIBONUCLEOPROTEIN-D; IMMEDIATE-EARLY GENES; RNA STABILITY; 3'-UNTRANSLATED REGION; HUR; IDENTIFICATION; EXPRESSION; KINASE; ACTIVATION;

Stability of A+U-rich element binding factor 1 (AUF1)-binding messenger ribonucleic acid correlates with the subcellular relocalization of AUF1 in the rat uterus upon estrogen treatment

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