One of the methods commonly used for manufacturing fructose 1,6-diphosphate is based on the enzymatic phosphorylation of glucose with inorganic phosphate using permeabilized brewer's yeast cells. Our results demonstrate that a substantial improvement in the yield of bioconversion can be achieved using fed-batch-grownSaccharomyces cerevisiaecells. Under an appropriate glucose and phosphate to cell ratio the efficiency of bioconversion reaches 70 of the theoretical value.
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