SummaryCoronavirus‐induced common cold and allergen‐induced rhinitis are characterized by nasal mucosal exudation of bulk blood plasma. The mucosal exudation process involves ‘flooding’ of the lamina propria with plasma‐derived binding proteins and it is possible that subepithelial inflammatory cytokines and mediators may be moved by the exudate to the mucosal surface. In this study, we have analysed cytokine levels in nasal lavage (NAL) fluids from non‐allergic subjects inoculated with coronavirus (n= 20) and from subjects with allergic (birch pollen) rhinitis subjected to additional allergen challenge (samples were obtained 35min post challenge) in the laboratory (n= 10). Ten of the 20 inoculated subjects developed common cold and 10 remained healthy. Interferon‐γ(IFN‐γ), interleukin‐1β (IL‐1β), granulocyte‐macrophage colony‐stimulating factor (GM‐CSF), IL‐4, and IL‐6 were analysed in unprocessed NAL fluids using immunoassays. The subjects who developed common cold had increased NAL fluid levels of IFNγ(P<0.05) that correlated well with the symptoms (P<0.001). IFNγdid not increase in subjects with allergic rhinitis. IL‐1β levels were similar in NAL fluids obtained from all inoculated subjects. In the subjects with allergic rhinitis NAL fluid levels of both IL‐1β and GM‐CSF were increased (P<0.05). GM‐CSF was not detected in common cold. IL‐4 and IL‐6 were not detectable in any of the NAL fluids. The present cytokines may not only emanate from superficial mucosal cells. By aiding plasma exudation subepithelial cytokines may potentially also be retrieved on the mucosal surface. Our study provides originalin vivodata supporting the notion that a TH‐1 profile of cytokines, notably IFNγ, is present in viral infection and further supporting the view that GM‐CS
展开▼
