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>Modification of T-Cell Antigenic Properties of Tetanus Toxoid by SDS-Page Separation. Implications for T-Cell Blotting
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Modification of T-Cell Antigenic Properties of Tetanus Toxoid by SDS-Page Separation. Implications for T-Cell Blotting
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机译：Modification of T-Cell Antigenic Properties of Tetanus Toxoid by SDS-Page Separation. Implications for T-Cell Blotting
Using Tetanus Toxoid (TT) as a model antigen the T-cell Blotting method was evaluated. Peripheral blood mononuclear cell (PBMC) cultures were stimulated by blotted nitrocellulose-bound TT or soluble TT. SDS-Poly-Acrylamide-Gel-Electrophoresis separated TT only induced proliferation in 20 of the PBMC cultures whereas proliferation was induced in 79 of the same cultures offered similar treated TT (except for the PAGE separation). When T-cell blotting was performed with TT separated in a SDS-agarose matrix, proliferation was induced in 80 of donors responding to soluble TT. The results show that SDS-PAGE alters the ability of TT to induce T-cell proliferation, possibly due to unpolymerized acrylamide binding to proteins during SDS-PAGE. The use of SDS-PAGE T-cell blotting in the screening for T-cell antigens must therefore be reconsidered. We suggest the use of SDS-Agarose Gel Electrophoresis as an alternative when doing T-cell blots.
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