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首页> 外文期刊>Molecular Endocrinology >Glycogen Synthase Kinase-3 beta Is Involved in Ligand-Dependent Activation of Transcription and Cellular Localization of the Glucocorticoid Receptor
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Glycogen Synthase Kinase-3 beta Is Involved in Ligand-Dependent Activation of Transcription and Cellular Localization of the Glucocorticoid Receptor

机译：Glycogen Synthase Kinase-3 beta Is Involved in Ligand-Dependent Activation of Transcription and Cellular Localization of the Glucocorticoid Receptor

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Glucocorticoids (GC) induce cell cycle arrest and apoptosis in different cell types and therefore are widely used to treat a variety of diseases including autoimmune disorders and cancer. This effect is mediated by the GC receptor (GR), a ligand-activated transcription factor that translocates into the nucleus where it modulates transcription of target genes in a promoter-specific manner. Glycogen synthase kinase-3 (GSK3) regulates GR response by genomic and nongenomic mechanisms, although the specific role of each isoform is not well defined. We used GSK3 pharmacological inhibitors and isoform-specific small interfering RNA to evaluate the role of GSK3 in the genomic regulation induced by GC. GSK3 inhibition resulted in the reduction of GC-induced mRNA expression of GC-induced genes such as BIM, HIAP1, and GILZ. Knockdown of GSK3 beta but not GSK3 alpha reduced endogenous GILZ induction in response to dexamethasone and GR-dependent reporter gene activity. Chromatin immunoprecipitation experiments revealed that GSK3 inhibition impaired the dexamethasone-mediated binding of GR and RNA polymerase II to endogenous GILZ promoter. These results indicate that GSK3 beta is important for GR transactivation activity and that GSK3 beta inhibition suppresses GC-stimulated gene expression. Furthermore, we show that genomic regulation by the GR is independent of known GSK3 beta phosphorylation sites. We propose that GC-dependent transcriptional activation requires functional GSK3 beta signaling and that altered GSK3 beta activity influences cell response to GC. (Molecular Endocrinology 26: 1508-1520, 2012)

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