Ultrastructural study of cholecystokinin-like immunoreactivity in endocrine cells of the insect midgut

J.C.Andriès; J.C.Beauvillain

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Ultrastructural study of cholecystokinin-like immunoreactivity in endocrine cells of the insect midgut

机译：Ultrastructural study of cholecystokinin-like immunoreactivity in endocrine cells of the insect midgut

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Electron-microscopic immunocytochemistry for the demonstration of CCK-like material in basal endocrine cells of the midgut ofAeschna cyanea, Locusta migratoria, Carausius morosusandPeriplaneta americanawas performed by use of the peroxidase-antiperoxidase procedure and the colloidal gold method. Immunoreactive cells appeared scattered among digestive and regenerative cells of the epithelium. Immunoreactivity was specifically detected over round to oval electron-dense granules whose size appeared rather different from species to species. Thus, the average size of 30 (d30) of the largest granules ranged from 312 nm inPeriplaneta americanato 159 nm inCarausius morosuswith intermediate values inAeschna cyanea(d30=195 nm) andLocusta migratoria(d30=225 nm).

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《cell and tissue research》 |1988年第1期|75-81|共页
作者
J.C.Andriès; J.C.Beauvillain;
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Laboratoire de Biologie Animale et Unité Associée au CNRS n∘ 148: Endocrinologie Comparée des Invertébrés, Université des Sciences et Techniques de Lille et Unité 156 INSERM;

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Ultrastructural study of cholecystokinin-like immunoreactivity in endocrine cells of the insect midgut

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