AbstractThe distribution of phosphorus between the soluble and colloidal phases in buffaloes' skimmed milk, and the effects of five heat treatments on it, was determined by the rennet coagulation technique. The original P in milk and radioactive32P incorporated into milk, bothin vitroandin vivo, were estimated for total and soluble phases by chemical and radioassay methods. The heat treatments consisted of pasteurisation (15 s at 71.8°C), pre‐warming (5 min at 96°C), sterilisation (15 min at 121.5°C), and cooling for 24 h at 4–6 and 0°C. When32P was addedin vitroabout 60 remained in the soluble phase; if incorporatedin vivo, about 39 was in the soluble phase, while the level of the original P was about 38. Pasteurisation, pre‐warming and sterilisation progressively decreased the soluble in‐vitro incorporated32P to about 58, 48 and 34, respectively. Cooling at 4–6 and 0°C altered the soluble in‐vitro incorporated32P to approximately 53 and 57, respectively, from about 64 in the raw milk. On the other hand, changes in partitioning of either in‐vivo incorporated32P or original P due to any of the five processing treatments were small, non‐uniform and not stati
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