A new control strategy was experimentally assessed on a laboratory scale by means of a two-stage fermentation for yeast production. The two-stage fermentation includes a batch culture to grow inoculum and a subsequent fed-batch culture. The strategy was based on controlling biomass concentration throughout the two-stage fermentation by manipulating the glucose feed rate. An L/A algorithm continuously tuned by ethanol concentration and respiratory quotient was used with constant external settings. The L/A controller performed satisfactorily throughout the two-stage fermentation and was able to move smoothly from one phase of operation to another. An average specific growth rate of 0.25 h−1and a cellular yield of 0.5 g·g−1were achieved during the fed-batch phase while over 40 g·l−1biomass was o
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