Optimization of Tissue Microarrays from Banked Human Formalin-Fixed Paraffin Embedded Tissues in the Cancer Research Setting

Tammy Sexton; Gregory L. Kucera; Edward A. LevineKounosuke WatabeStacey S. ONeill

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Optimization of Tissue Microarrays from Banked Human Formalin-Fixed Paraffin Embedded Tissues in the Cancer Research Setting

机译：Optimization of Tissue Microarrays from Banked Human Formalin-Fixed Paraffin Embedded Tissues in the Cancer Research Setting

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The tissue microarray (TMA) is a powerful tool for cancer biomarker discovery and validation. Tens to hundreds of samples can be evaluated simultaneously for molecular marker status at the protein or nucleic acid level. Although fully automated or semiautomated technologies for TMA creation provide excellent precision with respect to core transfer, they do not obviate the need for technical expertise to successfully generate high-quality TMA blocks and derivative sections. We have leveraged our expanding bank of formalin-fixed paraffin embedded paired tumor and normal tissues in our academic cancer center to provide a rich source of input material for cancer research TMAs. In this study, we report a stepwise optimization of TMA generation parameters, including paraffin wax selection, tempering protocol, and sectioning conditions, to achieve the best ribbon sectioning.

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《Biopreservation and biobanking》 |2019年第5期|452-457|共6页
作者
Tammy Sexton; Gregory L. Kucera; Edward A. LevineKounosuke WatabeStacey S. ONeill;
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作者单位

Wake Forest Baptist Comprehensive Cancer Center, Wake Forest School of Medicine;

Department of Internal Medicine, Section on Hematology and Oncology, Wake Forest School of Medicine;

Surgical Oncology Service, Department of General Surgery, Wake Forest School of MedicineDepartment of Cancer Biology, Wake Forest School of MedicineDepartment of Pathology, Wake Forest School of Medicine;

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原文格式 PDF
正文语种英语
中图分类细胞生物学;
关键词
tissue microarrays; TMA; FFPE; optimization;

Optimization of Tissue Microarrays from Banked Human Formalin-Fixed Paraffin Embedded Tissues in the Cancer Research Setting

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