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首页> 外文期刊>Mediterranean journal of nutrition and metabolism >Antioxidant and antibacterial evaluation of honey bee hive extracts using in vitro models
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Antioxidant and antibacterial evaluation of honey bee hive extracts using in vitro models

机译：Antioxidant and antibacterial evaluation of honey bee hive extracts using in vitro models

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Biological evaluation of honey bee hive extracts was estimated using a set of chemical and biological in vitro models. Extractions of bee hives were done in methanol and acetone solvents. Well-tolerated yield was observed in case of acetone solvent (12.34 ± 1.28 ) and methanol solvent (5.98 ± 0.94 ). Total phenolic and total flavonoid contents were found in the range of 359 ± 4.06-755 ± 6.03 and 0.952 ± 0.08-1.266 ± 0.04 mg/100 g dry extract, respectively. Antioxidant potential was tested using most reliable in vitro models, such as DPPH free radical scavenging model, lipid peroxidation inhibition in linoleic acid system and ABTS radical scavenging assay. The DPPH scavenging assay showed 28-55 radical scavenging, while the peroxidation inhibition in linoleic acid system showed low peroxidation inhibition 6-26 . TEAC value showed 4.12 ± 0.19 and 3.63 ± 0.5 muM TE/g for methanol and acetone extract, respectively. The antibacterial assay of methanol and acetone extracts showed good zone of inhibition in following order S. aureus (19.1 ± 1.15, 14.9 ± 0.53 mm) >B. sub-tilis (15.4 ± 0.07,13.1 ± 0.72 mm) >E. coli (13.8 ± 0.95, 9.9 ± 0.35 mm). Overall result demonstrated promising antioxidant and antibacterial potential of honey bee hive extracts.

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