AbstractSUP2(SUP35)is an omnipotent suppressor gene, coding for an EF‐1α‐like protein factor, intimately involved in the control of translational accuracy in yeastSaccharomyces cerevisiae.In the present study aSUP2gene analogue from yeastPichia pinuswas isolated by complementation of the temperature‐sensitivesup2mutation ofS. cerevisiae.The nucleotide sequence of theSUP2gene ofP. pinuscodes for a protein of 82·4 kDa, exceeding the Sup2 protein ofS. cerevisiaeby 6 kDa. Like theSUP2gene product ofS. cerevisiae, the Sup2 protein ofP. pinusrepresents a fusion of a unique N‐terminal part of a region homologous to EF‐1α. The comparison of amino acid sequences of the Sup2 proteins reveals high conservations (76) of the C‐terminal region and low conservation (36) of the N‐terminal part where, in addition, the homologous correspondence is ambiguous.Proteins related to the Sup2 ofS. cerevisiaewhere found inP. pinusand some other yeast species by the immunoblotting technique.The relation between the evolutionary conservation of different regions of the Sup2 protein and their functional significa
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