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Immunocytochemical identification of structures containing putative red pigment-concentrating hormone in two species of decapod crustaceans
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机译：Immunocytochemical identification of structures containing putative red pigment-concentrating hormone in two species of decapod crustaceans
By use of an antiserum raised against the Nterminal sequence pGlu-Leu-Asn-Phe..., common to red pigment-concentrating hormone (RPCH) ofPandalus borealisand three structurally similar insect neuropeptides, putative RPCH-immunopositive structures were revealed in the eyestalks ofCarcinus maenasandOrconectes limosusand in the brain and thoracic ganglion (TG) ofC. maenas.In the eyestalks, complete neurosecretory pathways were demonstrated, consisting of perikarya, axons and terminals in the neurohemal organ, the sinus gland (SG). InC. maenasapproximately 20 small RPCH cells are present as a distinct group adjacent to the medulla terminalis ganglionic X-organ (MTGXO, XO). They are morphologically different from the larger XO perikarya, which contain the crustacean hyperglycemic hormone (CHH). The occurrence of both neuropeptides in distinct neurosecretory pathways was ascertained by immunologic double staining (PAP/gold) or by analysis of consecutive sections. In addition, a group of two to four larger RPCH cells is located in the proximal part of the MT. InO. limosus, RPCH cells are found in the XO. Cells corresponding to the proximal MT cells ofC. maenaswere not found. In both species, a few more weakly staining immunopositive perikarya were observed in clusters of cell somata of the optic ganglia. It is uncertain whether these are connected to the SG.
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