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>Effect of cycloheximide and cordycepin on auxin-induced elongation andβ-glucan degradation of noncellulosic polysaccharides ofAvenacoleoptile cell wall
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Effect of cycloheximide and cordycepin on auxin-induced elongation andβ-glucan degradation of noncellulosic polysaccharides ofAvenacoleoptile cell wall
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机译:Effect of cycloheximide and cordycepin on auxin-induced elongation andβ-glucan degradation of noncellulosic polysaccharides ofAvenacoleoptile cell wall
The effect of cycloheximide (10−5M) and cordycepin (10−4M) used as protein and RNA synthesis inhibitors, respectively, on auxin action in noncellulosicβ-glucan degradation ofAvenacoleoptile cell wall was investigated. Both depressed auxin-inducedβglucan degradation of the cell wall as well as auxin-induced elongation and cell wall loosening, suggesting that the process ofβ-glucan degradation of the cell wall is closely associated with cell wall loosening and that auxin enhances the activity of an enzyme forβ-glucan degradation through de novo synthesis of RNA and protein but not through activation of the enzyme in situ. Kinetic study with the inhibitors showed that RNA metabolism involved inβ-glucan degradation was stimulated by auxin treatment of only 15 min while a longer lag phase (about 1 hr) existed for the synthesis of th
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