Fibroblast contraction of collagen gels requires activation of protein kinase C

Clyde Guidry

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Fibroblast contraction of collagen gels requires activation of protein kinase C

机译：Fibroblast contraction of collagen gels requires activation of protein kinase C

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AbstractFibroblasts stimulated to contract collagen gels with serum were completely inhibited by staurosporine, a broad spectrum kinase inhibitor. Further analysis demonstrated that staurosporine is potent (IC5017 nM), rapid in onset, and completely reversible. Complete inhibition of gel contraction was also observed with calphostin C (IC5048 nM), an inhibitor specific for protein kinase C (PKC). Similar effects were not observed with KT5926 or KT5720, inhibitors for myosin light chain kinase and cAMP‐dependent kinase, respectively. These data suggested that serum‐stimulated fibroblast contraction is dependent upon activation of PKC. This was also observed with fibroblast contraction stimulated with endothelin‐1, platelet‐derived growth factor, and transforming growth factor beta. PKC activated directly with low concentrations of phorbol ester was observed to stimulate fibroblast contraction of collagen gels, in some cases within 30 minutes of exposure. © 1993 Wiley

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《journal of cellular physiology》 |1993年第2期|358-367|共页
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Clyde Guidry;
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Fibroblast contraction of collagen gels requires activation of protein kinase C

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