AbstractBy using a system of agirose plating and agarose bead culture, it was possible to induce efficient somatic embryogenesis in protoplast‐derived calli of two rapeseed varieties, ‘Ceres’ and ‘Duplo’. Protoplasts were isolated from hypocotyls. For the initial protoplast culture a modified 8P medium was employed containing 2,4D (1.0 mg/l), NAA (0.1 mg/ 1), BAP (0.4 mg/l) and mannitol (7 ). After microcalli were obtained in four weeks, somatic embryos were induced by a two‐step method. This involved a modified MS medium containing 2,4D (3.0 mg/l) in the first step and no 2,4D, but BAP (3.0 mg/l) and GA3(0.1 mg/l) in the second. This procedure also secured plant r
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