Simultaneous flow cytometric measurement of antigen attachment to phagocytes and phagocytosis

DivisionofClinicalImmunologyDepartmentofMedicalTechnology; BiomedicalTechnologyResearchCenterNationalCenterforGeneticEngineeringandBiotechnology

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Simultaneous flow cytometric measurement of antigen attachment to phagocytes and phagocytosis

机译：Simultaneous flow cytometric measurement of antigen attachment to phagocytes and phagocytosis

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摘要

The current available assays cannot differentiate the stages of phagocytosis. We, therefore, established methods for concurrent detection of antigen attachment and engulfment by phagocyte using latex beads coated with lipopolysaccharide, rabbit IgG, and carboxyfluorescein diacetate succinimidyl ester. The generated beads were incubated with whole blood at 37°C for 1 hr and stained with PE-Cy5.5 anti-rabbit IgG antibody. By flow cytometry, attachment and phagocytic processes could be detected, simultaneously. The established method is a valuable tool for diagnosis of phagocytic disorder and study of molecules involved in phagocytosis.

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来源
《Journal of immunoassay and immunochemistry》 |2016年第6期|527-539|共13页
作者
DivisionofClinicalImmunologyDepartmentofMedicalTechnology; BiomedicalTechnologyResearchCenterNationalCenterforGeneticEngineeringandBiotechnology;
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作者单位

Chiang Mai University, Chiang Mai, Thailand;

National Science and Technology Development Agency at the Faculty of Associated Medical Sciences;

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原文格式 PDF
正文语种英语
中图分类医学免疫学;
关键词
attachment; engulfment; flow cytometry; opsonized beads; phagocytes; phagocytosis;

Simultaneous flow cytometric measurement of antigen attachment to phagocytes and phagocytosis

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