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Electrochemical immunoassay: Simple kinetic detection of alkaline phosphatase enzyme labels in limited and excess reagent systems
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机译：Electrochemical immunoassay: Simple kinetic detection of alkaline phosphatase enzyme labels in limited and excess reagent systems
AbstractKinetic measurement of an alkaline phosphatase enzyme label was performed with amperometric detection using conversion of substrate 4‐aminophenyl phosphate to the electroactive phenolic, measured at +125 to +200 mV tvs. Ag/AgCl. Calibration plots for thyroxine‐binding globulin (TBG) and cortisol were obtained by excess reagent and limited reagent immunoassays, respectively, which were comparable with those obtained using colorimetric detection. These provided a measurement range of 31–1000 μg/L for TBG and 100–2000 nM for cortisol. An antibody‐bound label was monitored in this system, and covering the electrode with a simple dialysis or polycarbonate membrane avoided electrode fouling. The kinetic mode of detection reduced assay time and helped to simplify the assa
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