Adenovirus-mediated expression of pig #alpha#(l, 3) galactosyltransferase reconstructs Gal #alpha#(l, 3) Gal epitope on the surface of human tumor cells

XING LI; GUO HONG XIA; JIAN FEIFANG HUANGLI HE GUO

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Adenovirus-mediated expression of pig #alpha#(l, 3) galactosyltransferase reconstructs Gal #alpha#(l, 3) Gal epitope on the surface of human tumor cells

机译：Adenovirus-mediated expression of pig #alpha#(l, 3) galactosyltransferase reconstructs Gal #alpha#(l, 3) Gal epitope on the surface of human tumor cells

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摘要

Gal #alpha#(l, 3) Gal (gal epitope) is a carbohydrate epitope and synthesized in large amount by #alpha#(l, 3) galactosyltransferase #alpha#(l, 3) GT enzyme on the cells of lower mammalian animals such as pigs and mice. Human has no gal epitope due to the inactivation of #alpha#(l, 3) GT gene but produces a large amount of antibodies (anti-Gal) which recognize Gal #alpha#(l, 3) Gal structures specifically. In this study, a replication-deficient recombinant adenoviral vector AdSsGT containing pig#alpha#(l, 3) GT cDNA was constructed and characterized. Adenoviral vector-mediated transfer of pig #alpha#(l, 3) GT gene into human tumor cells such as malignant melanoma A375, stomach cancer SGC-7901, and lung cancer SPC-A-1 was reported for the firsttune. Results showed that Gal epitope did not increase the sensitivity of human tumor cells to human complement-mediated lysis, although human complement activation and the binding of human IgG and IgM natural antibodies to human tumor cells were enhanced significantly after AdSsGT transduction. Appearance of gal epitope on the human tumor cells changed the expression of cell surface carbohydrates reacting with Ulex europaeus I (UEA I) lectins, Vicia villosa agglutinin (WA), Arachis hypogaea agglutinin(PNA), and Glycine max agglutinio. (SBA) to different degrees. In addition, no effect of gal epitope on the growth in vitro of human tumor cells was observed in MTT assay.

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来源
《Cell research》 |2001年第2期|116-124|共9页
作者
XING LI; GUO HONG XIA; JIAN FEIFANG HUANGLI HE GUO;
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作者单位

Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;

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收录信息美国《科学引文索引》(SCI);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类细胞生物学;
关键词
adenoviral vector; galactosyltransferase; Gal; alpha; (l; 3) Gal; gene expression; human tumor cell;

Adenovirus-mediated expression of pig #alpha#(l, 3) galactosyltransferase reconstructs Gal #alpha#(l, 3) Gal epitope on the surface of human tumor cells

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