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外文期刊>british journal of haematology
>A reversed activity staining procedure for detection of an acquired antibody against factor XIII in a girl with factor XIII deficiency
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A reversed activity staining procedure for detection of an acquired antibody against factor XIII in a girl with factor XIII deficiency
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机译：A reversed activity staining procedure for detection of an acquired antibody against factor XIII in a girl with factor XIII deficiency
Summary.A new method for detection of inhibitors against factor XIII is described. Agarose gel electrophoresis with normal platelet poor plasma as a factor XIII source included in the gel was combined with reversed activity staining. The procedure was applied to a 10‐year‐old girl with factor XIII deficiency where substitution therapy had failed. Plasma samples from the patient inhibited the factor XIII catalysed incorporation of the fluorescent monodansylthiacadaverine into casein at a position corresponding to the migration of γ‐globulins. Treatment with Sepharose‐bound protein A effectively adsorbed the factor XIII antibody in the patient'
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