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>Kinetic characterization of the effects of organic solvents on the performance of a peroxidase‐modified electrode in detecting peroxides, thiourea and ethylenethiourea
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Kinetic characterization of the effects of organic solvents on the performance of a peroxidase‐modified electrode in detecting peroxides, thiourea and ethylenethiourea
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机译：Kinetic characterization of the effects of organic solvents on the performance of a peroxidase‐modified electrode in detecting peroxides, thiourea and ethylenethiourea
AbstractHorseradish peroxidase (HRP) modified electrodes were constructed by entrapping the enzyme in an Eastman AQ 55D polymer. The biosensor detects butanone peroxide (BTP) as a substrate, and thiourea (THU) and ethylenethiourea (ETU) as HRP inhibitors. The kinetic parameters for this sensor in organic media such asImaxandK′mfor BTP detection andK′ifor ETU and THU detection were evaluated. The differences in the values of these parameters were explained in terms of the physico‐chemical properties of the organic reaction media. A possible solvent‐induced deviation of the biosensor behavior from Michaelis–Menten kinetics in the detection of ETU and THU was evaluated using the Hill analysis. The least deviation was observed in acetonitrile. Spectroelectrochemistry was also used to qualitatively assess solvent–HRP, BTP–HRP and inhibitor–H
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