Functional characterization of neurokinin‐1 receptors on human U373MG astrocytoma cells

Harald R. Eistetter; Ann Mills; Rachel BrewsterSami AlouaniChristine RambossonEric Kawashima

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Functional characterization of neurokinin‐1 receptors on human U373MG astrocytoma cells

机译：Functional characterization of neurokinin‐1 receptors on human U373MG astrocytoma cells

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AbstractThe neurokinin‐1 (NK‐1, substance P) receptor belongs to the class of seven transmembrane domain (7‐TM) receptors that interact with cellular effector systems via guanine nucleotide binding regulatory proteins (G‐proteins). In this study, coupling mechanisms of functional NK‐1 receptoirs endogenously expressed in a human astrocytoma cell line (U373MG) were analyzed. Stimulation with substance P (SP) resulted in 1) a rapid increase in inositol 1,4,5‐trisphosphate (IP3) synthesis; 2) a rise in cytosolic free calcium concentration (Ca2+); 3) induction of immediate early gene transcription as monitored byc‐fosandc‐junexpression; and 4) a significant increase in de novo DNA synthesis. Thus, the functional responses induced by stimulation of NK‐1 receptors on U373MG strongly correlate with those observed after treatment of primary astrocytes with SP and make U373MG cells a useful in vitro model system for the analysis of NK‐1 receptor function on astrocytes in vivo. ©

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《glia》 |1992年第2期|89-95|共页
作者
Harald R. Eistetter; Ann Mills; Rachel BrewsterSami AlouaniChristine RambossonEric Kawashima;
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正文语种英语
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Substance P receptor; IP3; Calcium; “Immediate early” genes; DNA synthesis;

Functional characterization of neurokinin‐1 receptors on human U373MG astrocytoma cells

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