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首页> 外文期刊>journal of cellular physiology >Zinc transport into endothelial cells is a facilitated process
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Zinc transport into endothelial cells is a facilitated process

机译：Zinc transport into endothelial cells is a facilitated process

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AbstractThe kinetics of zinc transport were examined by measuring the uptake of65Zn into cultured endothelial cells. This served as a suitable model for characterizing the transport of zinc across a biological membrane (i.e., the plasma membrane). The transport process was saturable under physiological conditions, which indicates a facilitating transport mechanism. Within the physiological range of zinc concentrations, the maximum zinc transport rate was 27 pmoles zinc/(min × mg protein) and it was half maximal at 4.1 μM zinc. Cadmium competitively inhibited zinc transport (Ki= 6.5 μM), while equimolar concentrations of copper and manganese were ineffectual. The rate of zinc transport was substantially reduced at lower temperatures and in the presence of sulfhydryl blockers (sodium iodoacetate and N‐ethylmaleimide). Inhibitors of energy metabolism (2,4‐dinitrophenol and sodium azide) failed to disrupt zinc transport. These results demonstrate that zinc transport into endothelial cells is a facilitated process (i.e., it is carrier mediated and energy‐independent). © 1992 Wiley

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