SUMMARYWe have compared the NZB/BINJ (/NJ) and NZB/BlPt (/Pt) mouse substrains with respect to a number of immunological and biochemical polymorphisms. In agreement with earlier findings by other workers, we detected histocompatibility antigen difference(s) between the two substrains. Serological analysis indicated that /NJ and /Pt expressed the same allotypes of H‐2, Thy‐1 and Lyt‐1 antigens, while they differed for the Lyt‐2 antigen. Amongst 15 biochemical polymorphisms, which we assayed, /NJ and /Pt differed for four, namely Pep‐3, Mup‐1, Gpd‐1 and Hbb. In addition, the two NZB substrains differed for the Hc marker (haemolyti
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