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Development of an NMR metabolomics-based tool for selection of flaxseed varieties

机译：Development of an NMR metabolomics-based tool for selection of flaxseed varieties

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摘要

Flaxseed is an important source of lignans and omega-3 fatty acids, compounds which present interest in human health with many applications in food industry. It is therefore necessary to precisely know the metabolite content in flaxseed. A metabolomic approach using NMR was developed to achieve this goal. Due to particular characteristics of flaxseed (high level in oil, high amount in mucilage, and integration of the phenolics into a macromolecule), the extraction procedure had first to be optimized using an experimental design, based on the extraction time, in a water bath or an ultrasound bath, alkaline treatment, defatting, and centrifugation temperature. This methodology was then applied to several flaxseed varieties classified in function of their content in omega-3 fatty acid. The main differences in semi-polar metabolites between these varieties concern compounds of the phenylpropanoid pathway. Hydroxycinnamic acid glucoside and lignan content increase when omega-3 fatty acid content decrease whereas flavonoid content increase in the same way of omega-3 fatty acids.

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