AbstractAddition of 50 μg/ml sodium ascorbate to confluent cultures of isolated rat calvarium bone cells resulted in a 21 increase in DNA production, a 50–60 increase in incorporation of 14Cproline into collagenous and noncollagenous proteins, and a 200 increase in alkaline phosphatase activity; under identical conditions, 35Ssulfate incorporation into proteoglycans (glycosaminoglycans) was not affected. These results suggest that ascorbate may be important in maintaining or stimulating the osteogenic phenotype of normal bone cel
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