The DNA damage and repair effects of yeast (Saccharomycescerevisiae) cells induced by lead (Pb) were tested in this study.yeast cells were cultured for 72 h at the following Pbconcentrations: 0 (controls), 1ppm (11.6μg.dl) and 8 ppm (92.6μg/dl), and 0.1 g kappa-selenocarrageenan (10000μg Se/g) used asrepair reagent was added directly to the yeast culture medium (100ml)for the repair assays. The percentage of damaged cells was observedfrom fluorescence microscope. Statistical analysis showed that thetwo lead doses damaged DNA structure of yeast cells significantly,and the higher concentration made more serious damage than that ofthe lower concentration. With the addition ofkappa-selenocarrageenan, DNA damage was repaired to some degree.Furthermore, the accumulation effects of DNA damage from the parentgeneration to the filial generation yeast cells was alsoinvestigated. At t he lower lead concentration, the accumulationeffects were more significant than that at the higher concentration.
展开▼
