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>Monoclonal antibody raised against murine IL-1 α peptide cross-reacts with a 60-kDa antigen in earlyDrosophila melanogasterembryo
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Monoclonal antibody raised against murine IL-1 α peptide cross-reacts with a 60-kDa antigen in earlyDrosophila melanogasterembryo
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机译：Monoclonal antibody raised against murine IL-1 α peptide cross-reacts with a 60-kDa antigen in earlyDrosophila melanogasterembryo
Whole-mounts ofDrosophilaembryos were stained with the monoclonal antibody Vmp 18, raised against the peptide 199–208 of murine interleukin 1/α. Immunofluorescence observations showed that the antibody cross-reacted with an antigenic determinant that changed in localization duringDrosophiladevelopment. In syncytialDrosophilaembryos, the antibody recognized an epitope localized on the nuclear envelope throughout mitotic division. As cellularization occurred, the fluorescence was mainly concentrated in the apical region of the blastoderm cells. Western blot analysis of wholeDrosophilaembryo extracts showed that the antibody recognized a 60-kDa protein in syncytial embryos and during germ band elongation. This suggests that the 60-kDa antigen undergoes dynamic redistribution during embryogenes
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