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>Distinct effects of phorbol esters and exogenous diacylglycerols in the induction of murine thymocyte proliferation
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Distinct effects of phorbol esters and exogenous diacylglycerols in the induction of murine thymocyte proliferation
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机译：Distinct effects of phorbol esters and exogenous diacylglycerols in the induction of murine thymocyte proliferation
AbstractMurine thymocytes were stimulated with the protein kinase C activating agents Phorbol‐12‐myristate‐13‐acetate (PMA) or a more physiological membrane permeant diacylglycerol (dioctanoyl‐sn‐glycerol, DiC8) in the presence or absence of exogenous lymphokines (rlL‐1β, rlL‐2). Whereas PMA directly induced reactivity to rlL‐2, DiC8 did not but had to synergize with the calcium ionophore lonomycin. Expression of the p55 chain of the IL‐2 receptor behaved similarly. In the absence of exogenous rlL‐2, thymocytes proliferated in response to a combination of lonomycin and PMA; however, replacing PMA by a single addition of DiC8 did not result in proliferation. Stimulation with lonomycin plus repeated addition of DiC8 induced a low level of thymocyte proliferation and further addition of rIL‐1β resulted in a significant increase. Purified immature (L3T4−Lyt2−) thymocytes behaved similarly, but showed an increased sensitivity to rIL‐1β. Taken together, the data support the idea that PMA and the more physiological diacylglycerols do not possess totally equivalent activities in lymphocyte sti
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