Maize (Zea maysL.) A188 calluses derived from leaf tissues ofin vitrogrown seedlings were initiated and maintained on Murashige and Skoog medium supplemented with 2 mg/1 2,4-dichlorophenoxyacetic acid (2,4-D). The calluses produced green leafy structures and subsequently plantlets upon transfer to N6 medium containing 2 mg/1 2,4-D and 0.1 mg/1 zeatin. An embryo-like structure with a green prominent coleoptile and a scutellum-like body was also obtained.
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