A proteomic approach was used to identify proteins differentially expressed in the plasma of mice following alternate day fasting. Male mice (C57B16, 9 months old) were randomly assigned to ad libitum (AL, n = 10) and calorie-restricted (IF, n = 10, AL on alternate day basis) groups for 4 weeks. After staining, the gels were imaged and differential protein expression patterns were interrogated using image analysis software. Spots showing a different expression level were identified through a comparison with 2D maps found in databases officially recognized (ExPASy). Master gels of AL and IF mice exhibited slightly different 2-DE patterns as only 14 spots out of 500 appeared differentially expressed: 12 spots were downregulated and identified as apolipoprotein E (APO-E), haptoglobin (HPT), alpha-1-antitrypsin (A1AT), pro-thrombin, and serum amyloid P component (SPA), whereas two spots were unidentified. In conclusion 4 weeks of alternate feeding results in a slight perturbation of the host plasma proteome. Of particular interest is the reduced plasma content in APO-E and acute-phase proteins HPT, Al AT and SPA that confirms a powerful anti-inflammatory effect of this dietary regimen.
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