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>Cytochemical demonstration of an enzymatic production of hydrogen peroxide on the surface of isolated thyroid capillaries
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Cytochemical demonstration of an enzymatic production of hydrogen peroxide on the surface of isolated thyroid capillaries
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机译：Cytochemical demonstration of an enzymatic production of hydrogen peroxide on the surface of isolated thyroid capillaries
By means of a cytochemical technique, hydrogen peroxide formation was located on the endothelial cell surface (predominantly the luminal aspect) of capillaries obtained by collagenase digestion of rat thyroid. The cyanide-insensitive H2O2formation required aerobic conditions and NAD(P)H as substrate. FAD could also stimulate the reaction, but not xanthine. The cytochemical reaction was blocked by a non-penetrating protein inhibitor. The observations are interpreted as evidence of a plasmalemma-bound H2O2-generating enzyme. The findings indicate that microvascular endothelial cells are involved in the release of activated oxygen species, which might have important pathophysiologic implications.
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